Berlin 2024 – wissenschaftliches Programm
Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe
BP: Fachverband Biologische Physik
BP 31: Protein Structure and Dynamics
BP 31.5: Vortrag
Donnerstag, 21. März 2024, 16:30–16:45, H 0112
NAP-XPS analysis of X-ray radiation damage to Proteins in Water — •Dorothea C. Hallier1, 2, 3, Jörg Radnik2, Paul M. Dietrich4, Harald Seitz1, 3, and Marc Benjamin Hahn2 — 1Fraunhofer Insitute for Cell Therapy and Immunology, Branch Bioanalytics and Bioprocesses, Potsdam, Germany — 2Federal Insitute for Materials Research and Testing BAM Berlin, Berlin, Germany — 3Univerity of Potsdam, Institute for Biochemistry and Biology, Potsdam Germany — 4SPECS Surface Nano Analysis GmbH, Berlin, Germany
X-ray photoelectron spectroscopy (XPS) was used to analyze the chemical damage of ionizing radiation to the single-stranded DNA-binding protein Gene-V Protein (G5P/GVP) and its most abundant amino acids. This protein plays a crucial role in maintaining the DNA metabolism, especially DNA replication, recombination and repair. XPS vacuum measurements were combined with near-ambient pressure (NAP) XPS measurements under water atmosphere to detect both direct and indirect radiation damage and to identify corresponding damage pathways. The exposure of proteins and aminoacids to x-rays leads to degradation i.e. via dehydrogenation, decarboxylation, dehydration and deamination. A strong increase of protein damage was observed in water as compared to vacuum.
Keywords: radiation damage; X-ray; ssDNA-binding protein; XPS; NAP XPS