Berlin 2024 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 36: Cell Mechanics II
BP 36.4: Vortrag
Freitag, 22. März 2024, 10:45–11:00, H 0112
An oblique plane light-sheet microscope for volumetric imaging of neural signals and in situ sample manipulation — •Achim Theo Brinkop1, Stefan Stöberl1, Florian Schorre1, Rebecca James1, Lena Glanz1, and Friedhelm Serwane1,2,3 — 1Faculty of Physics & Center for NanoScience, LMU Munich, Germany — 2Munich Cluster for Systems Neurology (SyNergy), Germany — 3Graduate School of Systemic Neuroscience (GSN), Munich, Germany
Many biophysical applications require high-speed volumetric imaging with open top access, e.g. long-term imaging of living organisms and sample manipulation. Existing microscope set-ups, however, are complex or tailored to specific applications. To investigate both mechanical and electrical properties of neural organoids, we designed and built a high-speed oblique plane microscope using one objective for illumination and detection. The set-up allows for imaging genetically encoded calcium and voltage indicators. At the same time, it is compatible with the use of magnetic actuators which can be mounted above the sample to probe the tissue’s mechanical properties in situ. First measurements show a signal-to-noise ratio on the order of 10 for Ca2+-imaging inside neural organoids at a single-cell level for exposure times of milliseconds. With this set-up, we aim to gain a deeper understanding of 3D organoid neuronal network formation and function.
Keywords: Light-sheet; Neural network; Organoid; Retina