Berlin 2024 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 8: Poster Session Ia

BP 8.13: Poster

Montag, 18. März 2024, 18:00–20:30, Poster C

Influence of perfluorocarbon on the structural changes of lipid monolayers and on protein adsorption — •Jaqueline Savelkouls, Christian Albers, Gordon Scholz, Eric Schneider, and Michael Paulus — Maria-Goeppert-Mayer-Straße 2, 44227 Dortmund

Perfluorocarbons (FCs) have high medical potential, serving as therapies in ophthalmology and respiratory diseases by replacing liquid FC ventilation and unsafe lung surfactant (LS) substitutes in the future [1]. We analysed the influence of the FC F-Decalin on the structural changes of model membranes of LS like anionic DPPA and zwitterionic DPPC monolayers at different initial surface pressures as well as on the adsorption of the surface-active proteins human serum albumin and lysozyme at beamline ID10 with a photon energy of 22 keV at the European Synchrotron Radiation Source (Grenoble, France). All samples were measured in situ at ambient temperature and pressure using a combined grazing incidence X-ray diffraction and X-ray reflectivity study. In summary, surface-active proteins adsorb to the lipid membrane either with and without a FC atmosphere. F-Decalin itself adsorbs to the interface between the head and tail groups of the lipid monolayer as well as to the hydrophobic regions of the lipid and protein. This leads to a compression of the lipid and protein layer. F-Decalin reduces the size of the crystalline domains, the surface tension of the monolayers and induces a fluidisation of the lipid monolayer. This effect is observed for monolayers with initially high surface tensions. [1] M. P. Krafft, DOI: 10.1002/pola.21937

Keywords: Perfluorocarbons; Protein adsorption; Langmuir films; X-ray reflectivity; grazing incidence X-ray diffraction