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BP: Fachverband Biologische Physik
BP 8: Poster Session Ia
BP 8.22: Poster
Montag, 18. März 2024, 18:00–20:30, Poster C
Formation of supported artificial Membranes of Lipid Raft Models by Physical Vapor Deposition — •Nancy Gomez-Vierling1, Daniel Saavedra1, Marco Soto-Arriaza2, Marcelo A. Cisternas3, Nicolás Moraga1, Tomás P. Corrales4, and Ulrich G. Volkmann1 — 1Instituto de Física and CIEN-UC, Pontificia Universidad Católica de Chile (UC), Santiago, Chile — 2Facultad de Medicina y Ciencia, Universidad San Sebastián, Santiago, Chile — 3Escuela de Ingeniería Industrial, Universidad de Valparaíso, Santiago, Chile — 4Departamento de Física, Universidad Técnica Federico Santa María, Valparaíso, Chile
The study focuses on advancing the development of rapid and cost-effective biosensors through the exploration of artificial membranes. The researchers, particularly from SurfLab UC, aim to create a Supported Lipid Bilayer (SLB) mimicking the Lipid Rafts model in cellular membranes. They employ the vapor-phase deposition method to determine optimal temperatures for evaporation rates of cholesterol, DOPC, and sphingomyelin molecules within a high vacuum chamber. The research delves into understanding the phases formed by these molecules at different temperatures. The obtained critical information guides the deposition of these molecules onto a silicon substrate, alongside DPPC molecules, to form self-assembling artificial bilayers resembling lipid rafts. The significance of this research lies in providing solvent-free alternatives for designing, fabricating, and storing phospholipid bilayer-based devices, including sensors and biomimetic devices. Acknowledgements: ANID Ph.D. Fellowships (NGV, DS, NM).
Keywords: Artificial Membranes; Lipid Raft Models; Supported Lipid Bilayers (SLBs); Membrane growth in High Vacuum; Ellipsometric Thickness Control