Berlin 2024 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 8: Poster Session Ia
BP 8.29: Poster
Montag, 18. März 2024, 18:00–20:30, Poster C
Elevating Understanding of Membranes: How Spectroscopic Techniques can draw from Super-Resolution Microscopy Principles — •Simone Ezendam1, Jonatan Alvelid1, Andrea Volpato2, and Ilaria Testa1 — 1Department of Applied Physics and Science for Life Laboratory, KTH Royal Institute of Technology, Stockholm, Sweden — 2Department of Women's and Children's Health and Science for Life Laboratory, Karolinska Institut, Stockholm, Sweden
Membranes and vesicles play pivotal roles in cellular processes, operating across various scales and intricately interacting with proteins. Super-resolution microscopy has transformed our understanding of membrane dynamics by providing higher resolution compared to traditional optical methods. However, gaining insights into the fast timescales governing translational and rotational diffusion necessitates spectroscopic techniques such as fluorescence correlation spectroscopy (FCS) and fluorescence anisotropy (FA). Because these techniques rely on fluorescence, they can leverage the same principles enabling super-resolution microscopy. An established example is STED-FCS. Recently, our lab introduced STARSS[1], extending time-resolved FA to large proteins. Expanding on these concepts, here, we propose the application of STED in FA for studying membranes.
[1] Volpato et al. Extending fluorescence anisotropy to large complexes using reversibly switchable proteins. Nat Biotechnol (2023). DOI: 10.1038/s41587-022-01489-7
Keywords: super resolution; fluorescence anisotropy; spectroscopy; membranes