Berlin 2024 – scientific programme

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BP: Fachverband Biologische Physik

BP 8: Poster Session Ia

BP 8.2: Poster

Monday, March 18, 2024, 18:00–20:30, Poster C

Imaging F-Actin arrangement via homo-FRET using 2D polarization fluorescence microscopy — Lukas Spantzel^1,2, Chen Sun³, Lena Jesse^1,2, Yunhao Mei^1,4, Yutong Wang^1,4, Shangjun Cheng^1,2,4, Mohammad Soltaninezhad^1,4, Michael Börsch^1,2, Rainer Heintzmann^1,4, Ivan G. Scheblykin³, Adrian T. Press^1,2, and •Daniela Täuber^1,4 — ¹Friedrich Schiller University, Jena — ²University Hospital Jena — ³Lund University, Sweden — ⁴Leibniz Institute of Photonic Technology, Jena, Germany

Polymicrobial infection affects the organization of F-Actin in the cytoskeleton and the cortex causing cell death. We use 2-dimensional polarization fluorescence imaging (2DPOLIM) to visualize the aggregation of phalloidin-dye labeled F-Actin via Förster Resonance Energy Transfer (homo-FRET). The homo-FRET efficiency observed from fibrillar structures in mouse embryonal fibroblasts agrees well with that of single fibrillar F-Actin synthesized from non-muscle Actin. Higher values are observed from other structures inside the cells, representing a more dense aggregation of the F-Actin in those regions.

Keywords: F-Actin; homo-FRET; fluorescence polarization; cytoskeleton