Berlin 2024 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 8: Poster Session Ia
BP 8.33: Poster
Montag, 18. März 2024, 18:00–20:30, Poster C
Stochastic catch-bond model of cell-cell adhesion mechanics and turnover — •Antonella Di Concilio Moschen1,2 and Benedikt Sabass1,2 — 1Department of Veterinary Sciences, LMU München — 2Department of Physics, LMU München
Catenins are proteins that mediate the binding between transmembrane molecules called cadherins and intracellular actin filaments in cell-cell adherens junctions. Mechanical forces generated by cytoskeleton are directly transmitted via αE-catenin to the membrane-localized E-cadherin/β-catenin complex.
It has been proposed based on in vitro experiments that this mechanotransduction mechanism is well described by a two-state catch bond between αE-catenin and F-actin. We aim to understand how the catch bond affects the mechanics and structural dynamics in vivo. Specifically we focus on differences between two distinct states of the adherens junctions called zonula-adherens and punctate-adherens junctions. By implementing a Gillespie algorithm to the system’s master equation, we construct a framework to quantitatively compare predictions from the above-mentioned two-state catch-bond model with results from FRET and FRAP experiments.
Keywords: Cell-cell adhesion; Self-stabilization; Master equation; Gillespie algorithm