Berlin 2024 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 8: Poster Session Ia
BP 8.36: Poster
Montag, 18. März 2024, 18:00–20:30, Poster C
Analysis of traction stress of iPSC derived heart muscle cells — •Bastian Malte Winter1, Fatemeh Abbasi2, Karthika Annamalai3, Karl Toischer4, and Timo Betz5 — 1Drittes Physikalisches Institut, Göttingen, Deutschland — 2Drittes Physikalisches Institut, Göttingen, Deutschland — 3Department of Cardiology and Pneumology, Göttingen, Deutschland — 4Department of Cardiology and Pneumology, Göttingen, Deutschland — 5Drittes Physikalisches Institut, Göttingen, Deutschland
During the maturation of animal tissue, their heart, and in particular their myocardium typically undergoes a change of stiffness. Furthermore, it is known that conditions like hypertrophy can also change the stiffness of the myocardium. Generally, changes in stiffness have an effect on force generation of single cells but to which extent this can be also applied to single cardiomyocytes is not well studied. Our goal is to investigate the effect of different substrate stiffnesses on the environment on force generation of single iPSC derived cardiomyocytes. For that, we seed cardiomyocytes on Polyacrylamide-gel (PAA-gel) of different stiffnesses and use Traction Force Microscopy to locate the forces generated by single cardiomyocytes in magnitude and direction. We show that generally an increase in stiffness also results in an increase in force production of single cardiomyocytes.
Keywords: Traction Force Microscopy; Cardiomyocytes; induced pluripotent stem cells