Berlin 2024 – scientific programme

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BP: Fachverband Biologische Physik

BP 8: Poster Session Ia

BP 8.37: Poster

Monday, March 18, 2024, 18:00–20:30, Poster C

Intracellular Mechanical Fingerprinting: Identifying the proteins controlling the intracellular active mechanics — •Noémie Veyret, Dorian Marx, Till Münker, and Timo Betz — Third institute of Physics, University of Göttingen, Germany

Over the past few years, the study of cell mechanical properties has allowed new insights on the understanding of biological processes and life complexity. According to previous work, intracellular mechanical properties can be narrowed down to a fingerprinting of only 6 parameters. Through the use of active and passive microrheology measurements via optical tweezers, frequency dependent viscoelastic properties and intracellular activity were found to vary for different cell types. The aim of this project is to find a correlation between changes in protein expressions and mechanical fingerprint of cells. To do so optical tweezers measurements will be performed during the differentiation process of induced Pluripotent Stem Cells (iPSC) into cell types derived from the three germ layers, namely neurons (ectoderm), skeletal muscles (mesoderm) and lung epithelia (endoderm). This measurement allows the characterization of the mechanics during the iPSC differentiation process. In parallel, the cell proteome will be studied using mass spectroscopy. Combining both, we hope to find the connection between proteins and their mechanical role, the intracellular "mechanome".

Keywords: Intracellular mechanics; Cellular fingerprinting; Microrheology; iPSC differentiation