Berlin 2024 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 8: Poster Session Ia
BP 8.39: Poster
Montag, 18. März 2024, 18:00–20:30, Poster C
Calcium-dependent flagellar adhessiveness of Chlamydomonas — •Marcel Schalling1, Rodrigo Catalan1, Marzieh Karimi2, and Oliver Bäumchen1 — 1University of Bayreuth, Experimental Physics V, 95447 Bayreuth, Germany — 2Max Planck Institute for Dynamics and Self-Organization, Am Faßberg 17, 37077 Göttingen, Germany
Calcium (Ca2+) signalling influences several flagellar processes in flagellated microbes, namely maintenance of the flagella and the regulation of their waveform and beat frequency.
The unicellular biflagellated microalga Chlamydomonas reinhardtii has been particularly used as a model organism to understand such processes.
Interestingly, recent evidence shows that intracellular Ca2+ influences the adhesion of C. reinhardtii of their flagella to abiotic surfaces [1].
Additionally, C. reinhardtii exhibits light-switchable flagellar adhesion [2], such that they adhere to surfaces under blue light and detach under red light.
Using single-cell micropipette force spectroscopy [3] in the presence of different concentrations of calcium in the medium, we study the effect of calcium on flagellar adhesiveness in different light conditions. Thereby, we aim at shedding light on the signal transduction pathway underlying light-switchable flagella adhesion.
[ 1 ] C. Fort et al., Journal of Cell Science (2021)
[ 2 ] C. Kreis et al., Nature Physics (2018)
[ 3 ] M. Backholm and O. Bäumchen, Nature Protocols (2019)
Keywords: calcium signalling; Chlamydomonas; flagellar adhesion; micropipette force spectroscopy