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CPP: Fachverband Chemische Physik und Polymerphysik
CPP 30: Charged Soft Matter, Polyelectrolytes and Ionic Liquids II
CPP 30.5: Vortrag
Mittwoch, 20. März 2024, 16:00–16:15, H 0107
Phase behavior and interactions of proteins in the presence of NaSCN — Mahnoush Madani1, Nele Koch1, Debes Ray2, Jan K. G. Dhont2, Kyongok Kang2, and •Florian Platten1,2 — 1HHU Düsseldorf, Faculty of Mathematics and Natural Sciences, Düsseldorf, Germany — 2FZ Jülich, IBI-4, Jülich, Germany
The state diagram of protein (lysozyme) solutions in the presence of sodium thiocyanate (NaSCN) is determined experimentally. Compared to NaCl, far lower salt concentrations are necessary to induce crystal formation and liquid-liquid phase separation (LLPS), respectively. These findings suggest a salt-specific effect of the SCN anion, strongly increasing net inter-protein attractions. Indeed, small-angle x-ray scattering experiments, used to determine the effective structure factor of the protein solutions, indicate a decrease of the second virial coefficient with SCN content, corresponding to increased net attractions.
Keywords: protein phase behavior; crystallization; LLPS; SAXS