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DS: Fachverband Dünne Schichten

DS 5: 2D Materials and their Heterostructures II: Graphene and Graphene Containing Heterostructures

DS 5.3: Talk

Tuesday, March 19, 2024, 10:00–10:15, A 053

Ultra-sensitive real-time detection of SARS-CoV-2 proteins with carbon nanomembrane/graphene field-effect transistor heterostructure — •Hamid Reza Rasouli¹, David Kaiser¹, Ghazaleh Eshaghi¹, Marco Reinhard², Alexander Rolapp², Dominik Gary³, Katrin Frankenfeld³, Thomas Weimann⁴, Michael Meister², and Andrey Turchanin¹ — ¹Institute of Physical Chemistry, Friedrich Schiller University Jena, 07743 Jena, Germany — ²Institut für Mikroelektronik- und Mechatronik-Systeme gemeinnützige (IMMS GmbH), 99099 Erfurt, Germany — ³Forschungszentrum für Medizintechnik und Biotechnologie (fzmb GmbH), 99947 Bad Langensalza, Germany — ⁴Physikalisch-Technische Bundesanstalt (PTB), 38116 Braunschweig, Germany

This study presents a novel approach to detect SARS-CoV-2 proteins based on graphene field effect transistors (GFET) functionalized via the van der Waals assembly with carbon nanomembranes (N3-CNM) enabling immobilization of antibodies for specific detection of the S- and N-proteins. A distinctive aspect of this approach is the simultaneous measurements of 15 GFETs integrated on a single chip with an automatic microfluidic system, facilitating the reliability for measuring the binding responses. The results demonstrate a detection limit down to the attomolar range with a dynamic response of 5 orders of magnitude. Furthermore, successful multiplex detection of S-protein and N-protein is demonstrated. The presented methodology paves the way towards highly sensitive, rapid, specific and multiplex detection of targets in various antibody-antigen systems.

Keywords: Graphene field-effect transistor; Carbon Nanomembrane; SARS-CoV-2 detection; Biosensor