Berlin 2024 – wissenschaftliches Programm
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DS: Fachverband Dünne Schichten
DS 5: 2D Materials and their Heterostructures II: Graphene and Graphene Containing Heterostructures
DS 5.3: Vortrag
Dienstag, 19. März 2024, 10:00–10:15, A 053
Ultra-sensitive real-time detection of SARS-CoV-2 proteins with carbon nanomembrane/graphene field-effect transistor heterostructure — •Hamid Reza Rasouli1, David Kaiser1, Ghazaleh Eshaghi1, Marco Reinhard2, Alexander Rolapp2, Dominik Gary3, Katrin Frankenfeld3, Thomas Weimann4, Michael Meister2, and Andrey Turchanin1 — 1Institute of Physical Chemistry, Friedrich Schiller University Jena, 07743 Jena, Germany — 2Institut für Mikroelektronik- und Mechatronik-Systeme gemeinnützige (IMMS GmbH), 99099 Erfurt, Germany — 3Forschungszentrum für Medizintechnik und Biotechnologie (fzmb GmbH), 99947 Bad Langensalza, Germany — 4Physikalisch-Technische Bundesanstalt (PTB), 38116 Braunschweig, Germany
This study presents a novel approach to detect SARS-CoV-2 proteins based on graphene field effect transistors (GFET) functionalized via the van der Waals assembly with carbon nanomembranes (N3-CNM) enabling immobilization of antibodies for specific detection of the S- and N-proteins. A distinctive aspect of this approach is the simultaneous measurements of 15 GFETs integrated on a single chip with an automatic microfluidic system, facilitating the reliability for measuring the binding responses. The results demonstrate a detection limit down to the attomolar range with a dynamic response of 5 orders of magnitude. Furthermore, successful multiplex detection of S-protein and N-protein is demonstrated. The presented methodology paves the way towards highly sensitive, rapid, specific and multiplex detection of targets in various antibody-antigen systems.
Keywords: Graphene field-effect transistor; Carbon Nanomembrane; SARS-CoV-2 detection; Biosensor