Berlin 2024 – scientific programme

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KFM: Fachverband Kristalline Festkörper und deren Mikrostruktur

KFM 12: Diamond and Related Dielectric Materials II

KFM 12.1: Talk

Wednesday, March 20, 2024, 09:30–09:50, TC 010

Diamond-based platforms for biochemical measurements of time-resolved clock cell signaling in response to external zeitgebers and coupling factors — •Rezvaneh Ghasemitabesh¹, Daniel Merker¹, Daniela Bertinetti², Friedrich W. Herberg², and Cyril Popov¹ — ¹Institute of Nanostructure Technologies and Analytics, Center for Interdisciplinary Nanostructure Science and Technology (CINSaT), University of Kassel, Germany — ²Department of Biochemistry, CINSaT, University of Kassel, Germany

Nowadays, techniques for immobilization of biomolecules for biosensor fabrication have been investigated on various diamond substrates due to their exceptional properties. This study focuses on the potential application of ultrananocrystalline diamond (UNCD) films as biosensor platforms for detection of biomolecules, such as neuropeptides (e.g. pigment dispersing factor (PDF)), secreted by clock neurons. To achieve an effective biosensor, calibration tests are initially conducted. These tests involve non-covalent and covalent immobilizations of green fluorescence protein (GFP) on nanostructured UNCD films. The results of non-specific binding of GFP to O-, H- and F-terminated UNCDs demonstrate that with decreasing GFP concentration, the fluorescence intensity is decreasing. Also, the immobilizations of various nanobodies against GFP show promising initial results. The long-term goal is to immobilize PDF-binding proteins for capturing PDF released from clock neurons and detecting it using a reporter complex composed of a binding protein and a fluorescent molecule.

Keywords: Diamond-based platforms; Ultrananocrystalline diamond (UNCD) films; Biosensor; Clock cell; Immobilization biomolecules