Berlin 2024 – scientific programme
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O: Fachverband Oberflächenphysik
O 6: Organic Molecules on Inorganic Substrates I: Adsorption & Growth
O 6.1: Talk
Monday, March 18, 2024, 10:30–10:45, MA 042
Unraveling Glycan Structures: ESIBD+STM Exploration of Stapled Conformations — •Dhaneesh Kumar1, Kelvin Anggara1, Manuel G. Ricardo2, Peter H. Seeberger2, and Klaus Kern1,3 — 1Max-Planck-Institute for Solid State Research, Stuttgart, Germany — 2Max-Planck-Institute of Colloids and Interfaces, Potsdam, Germany — 3École Polytechnique Fédérale de Lausanne, Switzerland
"Function follows form" holds a particular significance in the realm of biomolecules, where structural modifications can profoundly influence functionality. Recently, introduction of linkages or 'staples' at specific sites within a linear oligosaccharide (a.k.a. glycan) has been demonstrated to alter the biochemical properties of the glycan (1). Despite these advancements, the structural effect of these staples on glycan conformation has yet to be probed directly, leaving the bioactive conformations unknown.
Using Electrospray Ion Beam Deposition (ESIBD), we successfully transferred these stapled glycans onto a surface in vacuum, which are subsequently imaged individually by Scanning Tunnelling Microscopy (STM). By systematically varying factors such as stapling length and sites, we can visualize the possible conformational states of these stapled glycans. This integrated ESIBD+STM approach provides a complementary tool for studying biomolecular conformational states at the single-molecule level, overcoming limitations of conventional techniques.
(1) Ricardo M. G., et al.,J. A. Chem. Soc. (2022)
Keywords: Biomolecules; Scanning probe microscopy; Electrospray ionisation beam deposition; Glycans