Freiburg 2024 – scientific programme
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MO: Fachverband Molekülphysik
MO 25: Novel Experimental Approaches
MO 25.7: Talk
Friday, March 15, 2024, 12:30–12:45, HS 3044
Azobenzene based lipids as a tool to manipulate physiochemical properties of membrane mimetic systems via light — •Justin Hornbogen1, Ritu Rajender2, Stefan Kins2, Annette Meister3, David Glück4, Sandro Keller4, and Rolf Diller1 — 1Phys. Dept., RPTU, 67663 Kaiserslautern, GER. — 2Human Biol. Dept., RPTU, 67663 Kaiserslautern, GER. — 3Inst. Biochem. and Biotech., MLU, 06120 Halle, GER. — 4Inst. Molecular Bioscience (IMB), Univ. of Graz, 8010 Graz, AUT.
Regulation of physiological membrane properties is an auspicious approach towards the treatment of various illnesses, e.g. Alzheimer disease (AD). Azobenzene (AB) decorated lipids are used to manipulate membranes by photoinduced AB trans(E)/cis(Z)-isomerization. We present the photophysical switching behaviour of 18:0-azo-phosphatidylcholin incorporated into unilamellar phosphatidylcholine (POPC or DMPC) LUV's and glycodiisobutylene/maleic acid lipid particles (POPC- or DMPC-nanodiscs). In addition, we explore the physicochemical impact of AB isomerization by means of methods like (transient) UV/Vis spectroscopy, TEM, DLS and others. The self-assembling nanoparticles can serve as a model system to investigate biochemical functionality of membrane proteins in native-like biomembranes while altering membrane properties such as structure, thickness, lateral pressure, permeability etc. through a light stimulus. Ongoing purification and incorporation of APP and γ-secretase may reveal an influence of AB isomerization on the generation of pathogenic amyloid plaques and γ-secretase activity related to AD.
Keywords: azobenzene; membrane mimetics; self assembling; nanodiscs; spectroscopy