Freiburg 2024 – scientific programme
Parts | Days | Selection | Search | Updates | Downloads | Help
MO: Fachverband Molekülphysik
MO 7: Poster: Spectroscopy
MO 7.1: Poster
Tuesday, March 12, 2024, 17:00–19:00, Tent C
In Situ Hyperpolarized Benchtop NMR for Biomolecular Analysis at Natural Isotopic Abundance — •Jingyan Xu1,2,3, Raphael Kircher1,2,3, and Danila Barskiy1,2,3 — 1Johannes Gutenberg University, Mainz, Germany — 2Helmholtz-Institut, Mainz, Germany — 3Helmholtzzentrum für Schwerionenforschung, Darmstadt, Germany
Nuclear Magnetic Resonance (NMR) is a key noninvasive tool in fields ranging from research and industry to medicine. Benchtop NMR spectrometers have recently emerged as practical alternatives to traditional high-field NMR systems, especially for on-the-spot analysis and process monitoring. A notable challenge with these systems is the detection of low-abundance heteronuclei like 13C or 15N, hindered by low spin polarization resulting from weak interactions with the magnetic field. Our study introduces a novel hyperpolarization technique to overcome this limitation by integrating Signal Amplification by Reversible Exchange (SABRE) with a Spin-Lock Induced Crossing (SLIC) pulse sequence. Applied to various molecules, this method achieves up to 12% polarization for 15N and 0.4% for 13C, without needing sample transfer. Furthermore, our method allows for continuous hyperpolarization, paving the way for advanced applications in rapid 2D spectroscopy and relaxometry. This development offers a cost-effective, efficient means of detecting diluted chemicals using benchtop NMR, signaling a significant leap forward in diverse industrial and research applications.
Keywords: Benchtop NMR; In Situ Continuous Hyperpolarization; Heteronuclei; Natural abundance