Bonn 2025 – wissenschaftliches Programm
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K: Fachverband Kurzzeit- und angewandte Laserphysik
K 4: Photonics (3D Print) (joint session Q/K)
K 4.6: Vortrag
Montag, 10. März 2025, 18:15–18:30, HS V
3D printed micro-sized dark-field condenser by two photon polymerization — •Robert Horvat1, Leander Siegle1, Pavel Ruchka1, Michael Schmid2, Lukas Weseman3,4, and Harald Giessen1 — 14th Physics Institute, University of Stuttgart, Pfaffenwaldring 57, 70569 Stuttgart, Germany — 2Printoptix GmbH, Nobelstraße 15, 70569 Stuttgart, Germany — 3School of Physics, The University of Melbourne, Victoria 3010, — 4ARC Centre of Excellence for Transformative Meta-Optical Systems, School of Physics, The University of Melbourne, Victoria 3010, Australia
We demonstrate a miniaturized fully 3D printed dark field condenser for microscopy applications. Dark field microscopy is a simple but effective technique for contrast enhancement that allows imaging of transparent samples, useful in bio-medicine. Usually, microscope setups are bulky and costly. Our approach miniaturizes the system to the micro- and millimeter size, while allowing rapid prototyping and quick adaptation for individual system integration. We realise this by using two photon polymerization to 3D print two photoresists on both sides of a microscope glass slide. We first fabricate an annular ring aperture from a highly absorptive photoresist on one side of the glass slide with diameters between 300 and 2000 micrometers. Next we print a high numerical aperture lens within the same diameter range on the other side of the glass slide. We use the 3D printed dark field condenser to illuminate different samples, such as a USAF 1951 resolution test chart, and compare its performance to the typical bright field illumination.
Keywords: two photon polymerization; microscopy; additive manufacturing; 3D printing; imaging