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MO: Fachverband Molekülphysik
MO 23: Cluster and Nanoparticles I (joint session MO/A)
MO 23.3: Vortrag
Donnerstag, 13. März 2025, 11:45–12:00, HS XV
Optimized sample-delivery system for coherent-diffractive-imaging of proteins — •Stefanie Lenzen1,2, Lukas V. Haas1,3,4, Kevin Janson1, Amit K. Samanta1,3,4, and Jochen Küpper1,2,3,4 — 1Center for Free-Electron Laser Science (CFEL), Deutsches Elektronen-Synchrotron DESY, Hamburg — 2Department of Chemistry, Universität Hamburg — 3Department of Physics, Universität Hamburg — 4Center for Ultrafast Imaging, Hamburg
Determining the structure and dynamics of single native bio-nanoparticles, such as proteins, is still challenging. Several methods, including protein-crystallography and cryo-EM, focus on this challenge, but the biomolecule needs to be fixed, which might lead to structural disentegration, and the temporal resolution of these methods are limited. X-ray free-electron lasers (XFELs) provide ultrashort pulses, enabling diffraction before destruction, and a large number of photons, promising the observation of diffraction patterns off single nanoparticles. Aerodynamic-lens stacks were used to deliver collimated and focused particle beams for such experiments on large nanoparticles [1]. We optimized the sample injection and extended the use of particle beams toward smaller nanoparticles and protein complexes like apoferritin. This highlights the use of improved aerosolization methods together with optimized ALS injectors for small bio-nanoparticles. In addition, we present techniques for improved optical-scattering-based detection of proteins.
[1] Lena Worbs, Toward cryogenic beams of nanoparticles and proteins, Dissertation, Universität Hamburg (2022)
Keywords: optical scattering microscopy; single particle imaging; proteins