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Regensburg 2025 – scientific programme

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BP: Fachverband Biologische Physik

BP 14: Poster Session I

BP 14.1: Poster

Tuesday, March 18, 2025, 10:00–12:30, P3

The Role of Localized Metabolic Activity in Streptomyces Hyphae: An Agent-Based Approach — •Ricardo Santander1, Denis Iliasov2, Thorsten Mascher2, and Vasily Zaburdaev11Max-Planck Zentrum für Physik und Medizin, Kussmaulallee 2, Erlangen — 2Institut für Mikrobiologie, Zellescher Weg 20b, Dresden

The filamentous bacterium Streptomyces undergoes complex multicellular development characterized by hyphal growth and branching. Recent discoveries of LpdA-containing fluorescent foci within the hyphae suggest localized sites of elevated metabolic activity and ATP production. To investigate their influence on hyphal morphogenesis, we developed an agent-based model simulating key cellular components and their interactions.

Simulations reveal that localized ATP production and high consumption rates near the tips create spatial heterogeneity in metabolic activity. By adjusting model parameters, the model replicates typical growth patterns and hyperbranching phenotypes observed experimentally.

Our findings suggest that the spatial distribution of metabolic foci and localized ATP production influence Streptomyces morphology and multicellular organization.

Keywords: Simulation; multicellularity; morphogenesis; metabolism; ATP

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