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BP: Fachverband Biologische Physik

BP 14: Poster Session I

BP 14.22: Poster

Dienstag, 18. März 2025, 10:00–12:30, P3

Phase Transitions in Non-Hydrated DPPC Lipid Bilayers Deposited on Silicon: Effects of Dry Nitrogen Atmosphere and Thermal Cycling — •Nicolás Moraga¹, Daniel Saavedra¹, Nancy Gomez-Vierling¹, Marcelo A. Cisternas², María José Retamal³, and Ulrich G. Volkmann¹ — ¹Instituto de Física, Pontificia Universidad Católica de Chile, Santiago, Chile — ²Escuela de Ingeniería Industrial, Universidad de Valparaíso, Chile — ³Facultad de Ingeniería, Universidad Finis Terrae, Santiago, Chile

This study investigates the phase behavior of DPPC lipid bilayers in a non-hydrated state, deposited on silicon substrates, under conditions that are both experimentally innovative and highly relevant for applied science and fundamental research. By assembling these bilayers in vacuum and exposing them to dry nitrogen atmospheres, we present a novel platform that extends beyond classical hydrated systems, with significant implications for biosensing technologies. Through controlled thermal cycling using high-resolution ellipsometry, a technique offering exceptional sensitivity to subtle changes, we analyze phase transitions, uncovering the impact of hydration-free environments on lipid bilayer organization. These findings provide new insights into the thermal resilience of DPPC bilayers, highlighting potential phase stability domains. Additionally, this approach simulates extraterrestrial-like conditions where water is absent, underscoring the adaptability of lipid-based structures and advancing our understanding of molecular organization under vacuum and inert atmospheres. Acknowledgements: ANID Fellowships (NM, DS, NGV); Puente UC 2024-25.

Keywords: Non-Hydrated Lipid Bilayers; Thermal Cycling; Ellipsometry (VHRE); Biosensing in Dry Environments; Astrobiology and Extreme Conditions