Regensburg 2025 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 14: Poster Session I
BP 14.4: Poster
Dienstag, 18. März 2025, 10:00–12:30, P3
Infrared Hyperspectral Mapping of Biofilms Growing in Confinement — •Felix Hermann Patzschke1, Valentina Schmitz2, Rohan Karande2,3, and Frank Cichos1 — 1Leipzig University, Peter Debye Institute for Soft Matter Physics, Linnéstr. 5, 04103 Leipzig — 2Leipzig University, Institute for Biochemistry, Johannisallee 21-23, 04103 Leipzig — 3Helmholtz Centre for Environmental Research, Permoserstraße 15, 04318 Leipzig
Biofilms are microbial communities characterized by complex spatial organization and dynamic chemical composition. The formation and growth of biofilms in confined environments are of significant interest in fields such as medicine and bio-engineering. We seek to establish a clearer understanding of the interplay between physical confinement and microbial behavior by investigating whether specific quantifiable aspects of a cavity's geometry can influence the likelihood of biofilm initiation and the rate of its growth.
We utilize Photothermal Infrared (PTIR) hyperspectral microscopy to acquire infrared spectral data at sub-cellular spatial resolutions. Through spectral decomposition, we aim to map the chemical composition of biofilms and distribution of nutrients in space and time. Our results provide a detailed view of biofilm structure, metabolic activity, and growth dynamics in confined settings. This work underscores the potential of PTIR microscopy as an impactful tool for advancing the understanding of biological systems in scientifically and technologically significant domains.
Keywords: Biofilms; Mid-IR Spectroscopy