Regensburg 2025 – scientific programme
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BP: Fachverband Biologische Physik
BP 17: Poster Session II
BP 17.19: Poster
Tuesday, March 18, 2025, 18:00–20:30, P4
Real-time monitoring of fluctuations in ATP levels and mechanobiological signatures in living cells — •Albina Nizamieva and Matthias Weiss — Experimental Physics I, University of Bayreuth, Bayreuth, Germany
Living cells are genuine non-equilibrium systems with a typical energy turnover of roughly 108 times thermal energy in every second. This translates to about 107 ATP hydrolysis events per second, with which cells may fuel, for example, signaling cascades and/or contractions of the actomyosin cytoskeleton to probe and migrate on the substrate underneath. Here we have used fluorescent reporter molecules to quantify in living cells (1) the temporally fluctuating ATP levels, and (2) fluctuations of a key mechanosensory protein that connects cellular mechanics and signaling cascades. Our data reveal marked fluctuations on the scale of minutes and beyond, whereas short-term fluctuations appear to only report on fundamental and ubiquitous physico-chemical fluctuations that are rooted, for example, in the dyes’ photophysics and diffusional motion.
Keywords: bioimaging; physico-chemical fluctuations in cells; ATP; energy turnover of a cell