Regensburg 2025 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 17: Poster Session II
BP 17.21: Poster
Dienstag, 18. März 2025, 18:00–20:30, P4
Accessing local aggregation in phalloidin-stained Actin filaments using 2D Polarization Fluorescence Imaging — •Shangjun Cheng1,2,3, Yutong Wang1,2, Yunhao Mei1,2, Hossein Zarei Oshtolagh1,2, Lukas Spantzel1,3, Patrick Then1,4, Hans-Dieter Arndt1, Adrian T. Press1,3, Rainer Heintzmann1,2, and Daniela Täuber1,2 — 1Friedrich Schiller University Jena — 2Leibniz Institute of Photonic Technology, Jena — 3Jena University Hospital — 4Microverse Imaging Center, Jena, Germany
2-dimensional polarization-resolved fluorescence imaging (2DPOLIM) can discriminate between aggregated and non-aggregated protein forms independent of the sample's alignment by providing access to the full in-plane polarization properties of the sample. In combination with a semi-quantitative analysis of Förster Resonance Energy Transfer between similar fluorophores (homo-FRET) it can map the local aggregation in cells and tissue [1]. Actin assembly and disassembly is essential for cellular dynamics. A previous study has shown the direct link between infection and aggregation of F-Actin in hepatocytes [2]. Here, we present our speeded-up home-built 2DPOLIM setup [3] along with its calibration and image registration protocols allowing for an acquisition time in the range of a second. First results from application to the investigation of phalloidin-stained Actin filaments are presented. -- [1] R. Camacho, et al., Advanced Materials, 31, 1805671, 2019. [2] P. Martinac, et al., Infection, 47, S6-S7, 2019. [3] Y. Wang, et al., Klosters, Switzerland, January 2023. doi:10.13140/RG.2.2.35169.79204
Keywords: Bioimaging; Polarization; Förster Resonance Energy Transfer; Actin; Phalloidin-staining