Regensburg 2025 – scientific programme
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BP: Fachverband Biologische Physik
BP 17: Poster Session II
BP 17.22: Poster
Tuesday, March 18, 2025, 18:00–20:30, P4
Liquid-cell Scanning Transmission Electron Microscopy (STEM) of isolated mitochondria and respective Au labels — •Eric Lieberwirth1, Kevin Oldenburg2, Anja Schaeper3, Marcus Frank4, Ingo Barke1, Simone Baltrusch3, and Sylvia Speller1 — 1Institute of Physics & LLM, University of Rostock — 2ELMI-MV, University of Rostock — 3Institute of Medical Biochemistry and Molecular Biology, Rostock University Medical Center — 4Electron Microscopy Center, Rostock University Medical Center
In situ liquid-cell Scanning Transmission Electron Microscopy (STEM) holds the promise to observe biological organisms in a native state such as organelles, bacteria and eukaryotic cells [1,2]. In addition to acquisition of individual images, movies can be recorded during manipulation of tissue [3]. The potential radiation damage due to the transit of the electron beam through the sample is still under debate [4]. We imaged isolated mitochondria in Krebs-Ringer medium, and extracted imaging performance, and external features of radiation damage. We also study Au labels in the physiologic medium and show that atomic resolution of the nanoparticles is attainable. Such labelling is expected to increase resolution [1] and validate the presence of mitochondria in the STEM. One of the next challenges is validating the metabolic activity of the mitochondria during or upon the (S)TEM measurement.
[1] Kun He et al. (2019) J. Phys.: Condens. Matter 31 103001
[2] Frances M. Ross (2024) Micro. Tod. 32 17-22
[3] Elliot S. Pohlmann et al. (2015) Nano Lett. 15 2329-2335
[4] Yulian Wu et al. (2019) New J. Chem. 43 12548
Keywords: STEM; in situ; mitochondria; Au label; liquid-cell