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BP: Fachverband Biologische Physik

BP 17: Poster Session II

BP 17.23: Poster

Dienstag, 18. März 2025, 18:00–20:30, P4

Three-Axis Structured Illumination Lightsheet Microscopy — •Meelad Lalenejad and Alexander Rohrbach — University of Freiburg, Freiburg, Germany

Light-sheet microscopy (LSM) is known for increased image contrast and reduced photo-bleaching and toxicity since only those parts of the object are illuminated from the side that is in the focus of the objective lens. In addition, larger volumes are scanned plane-wise or line-wise by optimized laser beams, so LSM is significantly faster than point-wise scanning methods. However, for imaging a small number of cells, the spatial resolution is limited by the numerical aperture of the objective lens. We tackle the problem of limited resolution by combining holographically shaped illumination beams with three-axis interferometric arrangements. We use structured illumination microscopy (SIM) to obtain 3D super-resolved images in scattering media by generating interference fringes between every two beams from different illumination objective lenses.

Keywords: Light-Sheet Microscopy; Structured Illumination; Interference Contrast; SIM