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BP: Fachverband Biologische Physik

BP 17: Poster Session II

BP 17.24: Poster

Dienstag, 18. März 2025, 18:00–20:30, P4

Investigating Neutrophil dynamics using 200 Hz Rotating Coherent Scattering Microscopy — •Vera Obloh and Alexander Rohrbach — Lab for Bio- and Nano-Photonics, Department of Microsystems Engineering (IMTEK), University of Freiburg, Georges-Koehler-Allee 102, 79110 Freiburg, Germany

Neutrophils, the largest population of leukocytes in the human bloodstream, are initial responders in the rapid innate immune defense against most bacterial and fungal pathogens. They are activated before the complex humoral and lymphocyte-mediated processes of acquired immunity can effectively respond to an infection. To ensure effective defense, Neutrophils rapidly and efficiently move to areas of infection, based on highly dynamic processes of cytoskeleton reorganisation. Due to their ability to migrate rapidly and their availability and ease of cultivation, HL-60 Neutrophils are well suited for observations with Rotating Coherent Scattering (ROCS) microscopy, a novel 200 Hz label-free imaging technique with resolutions well below 200 nm. ROCS represents a powerful, high-speed alternative to fluorescence microscopy, especially for observations over thousands of frames. We represent first images and analyses of so far unseen details and dynamics of Neutrophil migration.

Keywords: Rotating Coherent Scattering microscopy; label-free superresolution; cell migration; neutrophils