Regensburg 2025 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 17: Poster Session II
BP 17.25: Poster
Dienstag, 18. März 2025, 18:00–20:30, P4
Characterisation of fluorescent dyes and their uptake by M2 cells using FLIM — •Jana Sütterlin1, Francisco Páez-Larios1,2, Lukas Harder1, Lea Klepsch3,4, Vivien Bachmann5, Antje Vollrath3,4, Paul Jordan5, Ulrich Schubert3,4, Oliver Werz5, Christian Franke1, and Christian Eggeling1,2 — 1Institute for Applied Optics and Biophoysics, Friedrich-Schiller-Universität Jena, Jena, Deutschland — 2Department of Biophysical Imaging, Leibniz-Institut für photonische Technologien e.V., Jena, Deutschland — 3Jena Center for Soft Matter, Friedrich-Schiller-Universität Jena, Jena, Deutschland — 4Institute for Organic and Macromolecular Chemistry, Friedrich-Schiller-Universität Jena, Jena, Deutschland — 5Department of Pharmaceutical and Medical Chemistry, Friedrich-Schiller-Universität Jena, Jena, Deutschland
Polymeric nanocarriers are used to incorporate active substances into cells, that otherwise would have limited bioavailability. To study the particle-cell-interaction, the nano-particles contain a fluorescent dye, which allows monitoring by fluorescence microscopy. Since a dye's fluorescence lifetime depends on its environment, the dye's release from the nanoparticle into the cellular cytosol can be evaluated temporally and spatially by fluorescence-lifetime-imaging (FLIM). To that end, lifetime behaviour of Nile Red, ATTO 665 and ATTO Rhodamine 3B is characterised under different solvent conditions mimicking different cellular compartments. By this, a comparison with FLIM data of live cell uptakes is possible, which can yield insights into the dynamic interaction of drug-loaded nanoparticles and their target cell.
Keywords: FLIM; fluorescence-lifetime-imaging; nano-particles; live cell imaging