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BP: Fachverband Biologische Physik

BP 17: Poster Session II

BP 17.26: Poster

Dienstag, 18. März 2025, 18:00–20:30, P4

MINFLUX-derived particle traces reveal Mean Back Relaxation to study active systems — •Deisel Tobias, Muenker Till, Vos Bart, and Betz Timo — Third Institute of Physics, Georg-August Universität Göttingen, Göttingen, Germany

Living systems like cells exhibit dynamics far from thermodynamic equilibrium. In order to study such non-equilibrium systems, we need to use analytical methods beyond the classical methods developed in statistical physics. In order to quantify the activity in a living, we have recently introduced the Mean Back Relaxation (MBR), which exploits a three-point probability function and is solely derived from passive measurements. A main hurdle in using the MBR in the requirement of particle trajectories with high temporal and spatial precision, that are sufficiently long to detect activity. In normal flourescence microscopy this is not possible to achieve because of probe bleaching. To overcome this, we measure the MBR using MINFLUX nanoscopy, which is able to track fluorescent particles at a spatio-temporal resolution in the order of nanometers at a frequency in the order of a few kHz. We explore the MBR of fluorescent particles in living cells and study its change under the influence of cytoskeletal inhibition.

Keywords: MINFLUX; Mean Back Relaxation