Regensburg 2025 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 17: Poster Session II
BP 17.45: Poster
Dienstag, 18. März 2025, 18:00–20:30, P4
Combining computational and experimental advances in microparticle traction force microscopy — •Bastian Kraus1, Simon Brauburger1, Tobias Walther2, Kerstin Göpfrich2, and Ulrich S. Schwarz1 — 1Institute for Theoretical Physics, Heidelberg University, 69120 Heidelberg, Germany — 2Center for Molecular Biology of Heidelberg University (ZMBH), Heidelberg University, 69120 Heidelberg, Germany
Traction force microscopy (TFM) infers cellular forces from the motion of fiducial markers embedded in soft elastic substrates. Over the last years, this approach has been extended to elastic microparticles, typically made from polyacrylamide. In contrast to flat substrates, this approach allows to infer forces either from the motion of embedded fiducial markers or from the deformation of the surface. Here, we compare these two different approaches from the viewpoint of elasticity theory and with computer simulations that include the image processing steps. We then apply the method to experimental data from DNA microbeads, for which one can implement markers for both bulk and surface deformations.
Keywords: Microparticle traction force microscopy; Computer simulations of elasticity theory; DNA microbeads; Volume or direct method; Surface or minimizing functional method