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BP: Fachverband Biologische Physik

BP 17: Poster Session II

BP 17.50: Poster

Dienstag, 18. März 2025, 18:00–20:30, P4

Regulation of plasma membrane tension through the actin cytoskeleton and hydrostatic pressure — •Yogishree Arabinda Panda and Elisabeth Fischer-Friedrich — Excellence Cluster Physics of Life, TU Dresden, Dresden, Germany

The plasma membrane and its associated proteins serve as a critical signaling hub, transmitting information between the extracellular environment and the intracellular space. It plays essential roles in regulating the intracellular ion content, the membrane potential and processes such as endocytosis and exocytosis. Consequently, the plasma membrane is central to many physiological processes including cell differentiation, migration, and proliferation. Recent studies have shown that the activity of many transmembrane proteins is influenced by mechanical tension in the plasma membrane. Despite its importance in cellular signaling, the mechanisms by which cells regulate membrane tension remain poorly understood. In this study, we investigate the regulation of plasma membrane tension in mitotically arrested cells using FLIM in conjunction with the membrane dye FlipTR. Specifically, we explore how components of the actin cytoskeleton, intracellular hydrostatic pressure, and cell shape contribute to both actual and apparent membrane tension.

Keywords: Plasma membrane tension; Cytoskeleton; FlipTR; Cortical Tension