Regensburg 2025 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 17: Poster Session II
BP 17.51: Poster
Dienstag, 18. März 2025, 18:00–20:30, P4
Single-cell physical phenotyping of blood and tissue biopsies — •Marketa Kubankova1, 2, Despina Soteriou1, 2, Martin Kräter1,2, and Jochen Guck1,2 — 1Max Planck Institute for the Science of Light, Erlangen, Germany — 2Max-Planck-Zentrum für Physik und Medizin, Erlangen, Germany
Deformability cytometry [1] is a microfluidic technique that allows the assessment of physical properties of single cells in a label-free and high-throughput manner, with up to 1000 cells analysed per second. Cell deformation and other physical phenotype parameters such as cell size and aspect ratio are obtained directly from brightfield cell images.
The diagnostic potential of deformability cytometry was previously demonstrated in various diseases [2, 3]. Here we show how deformability cytometry accurately discriminates between healthy and tumorous tissue in biopsies of mouse and human colons [4]. Cell deformation was a crucial parameter for the correct distinction of tumour tissue in colon cancer patients. Furthermore, we present new findings on how the physical properties of blood cells change during infectious diseases, and how they correlate with commonly used markers of infectious inflammation. Our findings pave the way for establishing deformability cytometry as a fast and marker-free diagnostic technique to sensitively detect pathological changes in solid and liquid biopsies.
[1] Otto et al., Nature Methods (2015), [2] Toepfner et al., Elife (2018), [3] Kubánková et al., Biophysical Journal (2021), [4] Soteriou and Kubánková et al., Nature Biomedical Engineering (2023)
Keywords: deformability cytometry; cell mechanics; immune cells; blood; biopsy