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BP: Fachverband Biologische Physik
BP 17: Poster Session II
BP 17.52: Poster
Dienstag, 18. März 2025, 18:00–20:30, P4
Mechanobiology of immune cell confined migration — •Fatemeh Abbasi1, Timo Betz2, and Eva Kiermaier1 — 1LIMES Institute, University of Bonn, Bonn, Germany — 2Third Institute of physics, University of Göttingen, Göttingen, Germany
In vivo, cells experience complex tissue environments and have to adjust their behavior and function based on their surrounding. Immune cells are the renowned examples. On their way from the bone marrow, where they are born, to the infection site, they have to cope with various physical challenges including geometrical confinement and different mechanical properties of the host tissues. To perform a successful confined migration, cells need to squeeze their nucleus, the most stiff and largest cell organelle, as well as reorganize their cytoskeleton. Despite the importance of this subject in immunology and pathology, it is still not well-understood how immune cells can adopt different nuclear morphologies and cytoskeleton organization while migrating through the small junctions and pores. Here, we use CFM (Confinement Force Microscopy), which was developed in the lab of Timo Betz, to confine immune cells in a 2.5D environment of various stiffness. We will study the role of centrosome, microtubule and nuclei morphology in innate immune cell confined migration. This study can help us to find out how nuclei and cytoskeletal organelles facilitate immune cell migration through confined microenvironments of different mechanical properties. Simultaneous measurement of the cell forces on the microenvironment will enable us to find out the mechanobiology of immune cell confined migration.
Keywords: Immune cell; cell mechanobiology; Cell confinement; Cell migration