Regensburg 2025 – scientific programme

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BP: Fachverband Biologische Physik

BP 17: Poster Session II

BP 17.58: Poster

Tuesday, March 18, 2025, 18:00–20:30, P4

Soft-landing Electrospray Ion Beam Deposition (ES-IBD) allows integration of native mass spectrometry and cryoEM to investigate membrane protein structure and function — •Carl von Hallerstein, Sophie Lawrence, Tarick El-Baba, Stephan Rauschenbach, and Carol Vivien Robinson — Kavli Institute for Nanoscience Discovery, University of Oxford, UK

Electrospray Ion Beam Deposition (ES-IBD) is an emerging sample preparation for the imaging of molecules (Esser et al. 2022, Faraday Discussions). Recently, using native mass spectrometry (nMS), the deposition and cryo-electron microscopy (cryoEM) imaging of soluble proteins was demonstrated (Esser et al. 2024, Sci. Adv.).

Here, we apply ES-IBD + cryoEM to membrane proteins, which only retain their native state while encased in lipid membranes or membrane-mimetics such as detergents or nanodiscs. ESIBD+cryoEM yields valuable information on lipid and surfactant interaction as well as hydration of the membrane protein.

Keywords: cryoEM; Native Mass Spectrometry; Membrane Proteins; Structural Biology