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BP: Fachverband Biologische Physik
BP 17: Poster Session II
BP 17.77: Poster
Dienstag, 18. März 2025, 18:00–20:30, P4
Imaging cell mechanics of retina organoids using an oblique plane light-sheet microscope — •Achim Theo Brinkop1,2, Florian Schorre1, Stefan Stöberl1, Elijah R. Shelton1, Teresa Rogler1,2, Michael Frischmann1,2, Marie Lackmann1, Kaustav Goswami1, Alexander Zangl1, Mythili Padavu1, and Friedhelm Serwane1,2,3 — 1Faculty of Physics & Center for NanoScience, LMU Munich, Germany — 2Institute of Biophysics, Ulm University, Germany — 3Munich Cluster for Systems Neurology (SyNergy) & Graduate School of Systemic Neuroscience (GSN), Munich, Germany
Retina organoids have become a powerful testbed for studying retina formation and neuronal development. Our current measurements of the creep compliance in retina organoids with magnetic droplets point towards soft glassy rheology of developing retinal tissue at second to hour timescales. As a next step, we explore whether the motion of cells agrees with predictions for glassy materials. For this purpose, we built a custom oblique plane microscope for long-term volumetric imaging of the cell movements during organoid development. Using a processing pipeline based on open-source python packages (Cellpose3, Ultrack), we segment and track individual cells. The tracks allow us to quantify cell dynamics and compare these with existing models for glassy materials. In the future, we will integrate magnetic droplet compliance measurements with volumetric imaging in one set-up to simultaneously probe organoid mechanics in situ. Combining tissue mechanics measurements with cell dynamic recordings, we aim to shed light on the mechanical cues that guide retina formation.
Keywords: Light-sheet; Organoid; Retina; Cell dynamics; Glassy material