Regensburg 2025 – scientific programme
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BP: Fachverband Biologische Physik
BP 17: Poster Session II
BP 17.83: Poster
Tuesday, March 18, 2025, 18:00–20:30, P4
Quantitative Analysis of Cell Detachment Using Optical Diffraction Tomography and Instance Segmentation — •Polina Malova1,2, Kyoohyun Kim1, and Jochen Guck1,2 — 1Max Planck Zentrum für Physik und Medizin — 2Max Planck School Matter to Life
Cell division is a fundamental biological process essential for growth, reproduction, and regeneration in all living matter. However, despite its critical role in the cell cycle, mitosis is not yet entirely understood. Mitotic rounding has proven to be a widely occurring phenomenon, the underlying physical processes of which are not fully researched. To investigate this process, we used the optical diffraction tomography (ODT) technique - a non-invasive approach for acquiring such parameters as refractive index (RI), dry mass, volume, and dry mass density. By applying instance segmentation algorithms, we explored the underlying mechanical phenomena of mitotic cell rounding by inducing interphase cell detachment with the introduction of cytoskeletal perturbations by inhibiting Rho-associated kinase (ROCK) and silencing Lymphocyte-oriented and Ste20-like kinases (LOK/SLK). Although there were distinct differences in rounding dynamics over time, our findings showed a delay of detachment in both conditions in comparison to control. The achieved results in this work set the ground for a better perspective of the mechanisms of underlying cytoskeletal changes in the cell during detachment.
Keywords: cell detachment; cytoskeleton; optical diffraction tomography; instance segmentation; mitotic rounding