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BP: Fachverband Biologische Physik

BP 18: Tissue Mechanics

BP 18.3: Vortrag

Mittwoch, 19. März 2025, 10:15–10:30, H44

Spatiotemporal Analysis of Active Deformation of Patient-derived Colon Cnacer — •Shogo Nagai¹, Ryo Suzuki², Go Yamakawa³, Akihisa Fukuda³, Hiroshi Seno³, and Motomu Tanaka^1,4 — ¹Physical Chemistry of Biosystems, Heidelberg University, Heidelberg, Germany — ²Department of Biosciences and Informatics, Keio University, Tokyo, Japan — ³Department of Gastroenterology and Hepatology, Kyoto University Graduate School of Medicine, Kyoto, Japan — ⁴Center for Integrative Medicine and Physics, Kyoto University, Kyoto, Japan

Biomedical cancer research has relied on the investigation of fixed cells and tissues, in which the non-equilibrium dynamics of cancer have been largely overlooked. Extending the recent studies shedding light on the dynamics of the isolated cells, spatiotemporal analysis of cancer on a multicellular level is expected to reveal the dynamic mechanisms of cancer progression.

In this study, the time evolution of active deformation of growing colorectal cancer organoids (miniaturized organ model) was quantitatively evaluated by the Fourier expansion. Thereby, the larger deformation of malignant genetic mutated organoids was extracted, which was attributed to the slow effective viscoelastic relaxation. The simulation of the double-cell stage indicated the characteristic dynamics of organoids could be related to cell-cell junctions. In addition, biomedical evaluations showed lower cell-cell junctions of malignant colorectal cancer on a protein and RNA level.

Keywords: Spatiotemporal analysis; Tissue dynamics; Cancer organoids; Cell-cell junctions