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BP: Fachverband Biologische Physik
BP 18: Tissue Mechanics
BP 18.5: Vortrag
Mittwoch, 19. März 2025, 10:45–11:00, H44
Bridging the gap between single cell and tissue mechanics — •Mathilde G. Lettinga1, Antje Garside1, Vaibhav Mahajan1, Franziska Baenke2, Valeria Lozovanu2, Daniel Stange2, Ingolf Sack3, and Anna V. Taubenberger1 — 1Center for Molecular and Cellular Bioengineering (CMCB), BIOTEC, Dresden University of Technology, Germany — 2Department of Visceral, Thoracic and Vascular Surgery, University Hospital Dresden, Germany — 3Department of Radiology, Charité - Universitätsmedizin Berlin, Germany
Tumours exhibit altered biophysical properties across spatial scales. Compared to healthy tissue, solid tumours are typically stiffer, while individual cancer cells are more compliant. The increased tissue stiffness can partly be attributed to the extracellular matrix. However, the contributions of single cell mechanics and collective cell behaviour to the emergent tissue properties remain unclear.
To bridge this gap between single cell and tissue mechanics, we have established a 3D in vitro tumour model, based on patient-derived colorectal liver metastasis organoids grown in hydrogels mimicking the extracellular matrix. Cells retrieved from dissociated organoids were mechanically characterised with real-time deformability cytometry and AFM. These data were benchmarked to the morphometric and mechanical properties of intact organoids, which were assessed in situ by confocal and Brillouin microscopy. The bulk mechanical properties of our model system were investigated using tabletop magnetic resonance elastography. Our data contribute to a better understanding of the mechanical coupling between single cells and tissues.
Keywords: Organoids; Microtissue; Cancer