Regensburg 2025 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 18: Tissue Mechanics
BP 18.6: Vortrag
Mittwoch, 19. März 2025, 11:00–11:15, H44
Exploring glassy dynamics in retina organoids through time-series imaging — •Alexander Johann Zangl1, Achim Theo Brinkop1,2, Elijah R. Shelton1, Marie Lackmann1,2, Teresa Rogler1,2, and Friedhelm Serwane1,2,3 — 1Faculty of Physics & Center for NanoScience, LMU Munich, Germany — 2Institute of Biophysics, Ulm University, Ulm, Germany — 3Munich Cluster for Systems Neurology (SyNergy) & Graduate School of Systemic Neuroscience (GSN), Munich, Germany
Quantifying cell dynamics and the mechanical forces guiding such movements can provide crucial insights for understanding tissue development and disease progression. Stem cell derived neuronal organoids provide an accessible system for studying nervous tissue development in the laboratory. Recently, magnetic droplet based mechanical measurements in retina organoids revealed a weak power-law scaling in the mechanical properties, suggesting that the retinal tissue is a glassy material. While the mechanical observations are consistent with predictions of soft glassy rheology, whether the movements of the individual cells making up those tissues are also in agreement with a glassy material is still unknown. Using confocal fluorescent time-series imaging, we observe the movements of nuclei in the forming retina. By tracking cells, we can determine whether cellular movements also point to the retina as a solid-like material just above a glass transition. We characterize the cell dynamics of the tissue by analysing the scaling of the mean-square displacements of the nuclei. This will help us understand how mechanical cues guide retina formation.
Keywords: Organoid; Retina; Glassy dynamics; Cell tracking; Jamming