Regensburg 2025 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 19: Membranes and Vesicles II
BP 19.4: Vortrag
Mittwoch, 19. März 2025, 10:15–10:30, H46
Vesicle to bicelle decomposition can be correlated with the lipid’s main phase transition: a direct evidence using chain-deuterated lipid — •Carina Dargel1,2, Lara H. Moleiro2, Aurel Radulescu3, Tim J. Stank2, and Thomas Hellweg2 — 1University of Münster, Institute of Physical Chemistry, Münster, Germany — 2University of Bielefeld, Physical and Biophysical Chemistry, Bielefeld, Germany — 3FZ Jülich, Jülich Centre for Neutron Science (JCNS) at Heinz Maier-Leibnitz Zentrum (MLZ), Garching, Germany
Mixtures of the phospholipid 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and the saponin β-aescin form bicelles above a critical saponin concentration. Modification of the membrane’s phase state by temperature increase induces a structural growth of the bicelles resulting in membrane-like DMPC-aescin aggregates or mixed small unilamellar vesicles.
The temperature-induced transition is fully reversible, independent of the aescin content. Furthermore, the decomposition of the mixed vesicles back to bicelles shows a prominent hysteresis effect, which is correlated with the main phase transition temperature Tm of the lipid. This correlation was demonstrated for the first time by taking advantage of the shift of the membrane’s Tm due to chain-deuteration and thus the use of d54-DMPC by both turbidimetry and small angle neutron scattering (SANS)[1].
[1] Dargel et al. (2025), Journal of Colloid and Interface Science, 679, 209-220.
Keywords: bicelle; (chain-deuterated) lipid; saponin; main phase transition temperature; SANS