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BP: Fachverband Biologische Physik
BP 20: Statistical Physics of Biological Systems I (joint session BP/DY)
BP 20.8: Vortrag
Mittwoch, 19. März 2025, 17:15–17:30, H44
Cellular morphodynamics as quantifiers for functional states of resident tissue macrophages in vivo — •Miriam Schnitzerlein1,2, Eric Greto3,4, Anja Wegner3,4, Anna Möller3,4, Oliver Aust3,4, Oumaima Ben Brahim3,4, Stefan Uderhardt3,4, and Vasily Zaburdaev1,2 — 1Department of Biology, Friedrich-Alexander-Universität Erlangen-Nürnberg (FAU) — 2Max-Planck-Zentrum für Physik und Medizin, Erlangen — 3Department of Medicine 3 - Rheumatology and Immunology, FAU und Universitätsklinikum Erlangen — 4Deutsches Zentrum für Immuntherapie, FAU
Resident tissue macrophages (RTMs) perform essential tasks such as clearing cellular debris to ensure tissue homeostasis. Such actions are accompanied by morphological changes in cell shape which reflect their functional states. Until now, RTMs were mostly studied in vitro, even though their dynamic behaviour in vivo is fundamentally different.
We employed a high-resolution, intravital imaging protocol to generate dynamic data of in vivo peritoneal RTMs of mice. Next we built a custom image processing pipeline to assess RTM morphodynamics via a set of human-interpretable cell shape and size features. Those features could quantitatively and also qualitatively differentiate between cells in different activation states. Furthermore, we showed that unperturbed RTMs exhibit a wide range of morphodynamical phenotypes, constituting a naive morphospace of behavioural motifs. Analysing cells challenged by chemical stimulations or due to aging gave us insights into how RTMs respond and adapt to inflammatory stimuli.
Keywords: Cell morphology and dynamics; Immune cells; Resident Tissue Macrophages (RTMs); Image analysis; Intravital Imaging