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BP: Fachverband Biologische Physik

BP 22: Bioimaging

BP 22.4: Vortrag

Mittwoch, 19. März 2025, 16:00–16:15, H46

Visualising immune cell interactions in lymph nodes — •Anna Schepers¹, Joannah Fergusson¹, Helena Coker¹, Robert Kochl², and Marco Fritzsche¹ — ¹Kennedy Institute of Rheumatology, Oxford, UK — ²King’s College London, UK

The inherently multiscale immune response is regulated by diverse cell interactions, relying on cues from tissues down to single cells and subcellular structures. The intricate dynamics of the immune system present challenges for the observation of the immune response. A technological advance has been achieved with the introduction of lattice light sheet microscopy (LLSM), allowing fast and gentle imaging of live samples while achieving subcellular resolution. By complementing LLSM-based volumetric imaging with advanced sample handling of ex vivo tissue samples and perfusion imaging chambers, we provide a system that preserves critical physiological complexity. The perfusion system ensures oxygen and nutrient supply to maintain and sample viability while, at the same time, enabling imaging of the perfused samples. We show that in our setup, we can follow single cells and their interactions in volumes several cell layers deep in living samples within their environment, providing nuanced insights into the immune response.

Keywords: immune cells; cell interactions; lattice light sheet microscopy; 4D microscopy