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BP: Fachverband Biologische Physik
BP 22: Bioimaging
BP 22.7: Vortrag
Mittwoch, 19. März 2025, 17:00–17:15, H46
Lysosomal activity in response to incubation of pristine and functionalized carbon nanodots — •Carla Sprengel1, Céline David2, Lena Berning2, Cathrin Nollmann1, Björn Stork2, and Thomas Heinzel1 — 1Condensed Matter Physics Laboratory, Heinrich Heine University, Düsseldorf, Germany — 2Institute of Molecular Medicine I, Medical Faculty and University Hospital Düsseldorf, Heinrich Heine University, Düsseldorf, Germany
Fluorescent carbon nanodots (CNDs) have emerged as promising carriers for drug delivery systems due to their high biocompatibility and functionalizability. We could not find an influence of CNDs on cellular lysosomal functions, as characterized via the cathepsin B and L activity and autophagic markers p62 and LC3B-II, even under high CND concentrations. Functionalization of CNDs with branched polyethylenimin (bPEI) as a model drug conjugate leads to a greater accumulation of bPEI-CND compounds within lysosomes compared to native CNDs. Here, changes in the lysosomal size and function can be explained exclusively by bPEI. This leads us to conclude that CNDs are highly efficient and inert carriers for delivering functional molecules into lysosomes as target while minimizing lysosomal escape and therefore preventing unintended side effects on other cell organelles.
Keywords: Nanoparticles; Drug Delivery Systems; Functionalization; Fluorescence; Lysosomes