Regensburg 2025 – wissenschaftliches Programm

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BP: Fachverband Biologische Physik

BP 25: Cell Mechanics II

BP 25.1: Hauptvortrag

Donnerstag, 20. März 2025, 09:30–10:00, H44

Cellular morphodynamics on microlanes – mechanistic models and simulation based inference — •Joachim O. Rädler — Faculty of Physics and Center for NanoScience, Ludwig-Maximilians-Universität München, Geschwister-Scholl-Platz 1, D-80539 Munich, Germany

Cell adhesive micro-pattern are a versatile platform to study eukaryotic motion as they provide defined geometric boundaries and controlled surface chemistry. We present time-lapse studies of cells navigating on 1D micro-lanes as well as ring-shaped micro-lanes and dumbbell pattern. Cell motion is tracked and the statistics of single cell behavior is analyzed using a mechanistic model based on a cytoskeleton force balance. The theory reproduces migratory multistability, i.e. the existence of distinct states of cell motion, as well as the experimentally observed characteristics of oscillations and probabilities of state transitions. The mechanical model predicts the occurrence of universal relations conserved across different cell lines such as the adhesion-velocity relation and the universal correlation between speed and persistence (UCSP). We highlight the unique opportunity of standardized 1D assays to validate theory based on statistical measures from large data of trajectories and discuss the potential of simulation based inference to obtain measures of cytoskeleton parameters that control migratory behavior.