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BP: Fachverband Biologische Physik
BP 7: Single Molecule Biophysics
BP 7.1: Hauptvortrag
Montag, 17. März 2025, 15:00–15:30, H44
Single-molecule dynamic structural biology with Graphene Energy Transfer — •Philip Tinnefeld1, Alan Szalai1, Giovanni Ferrari1, Lars Richter1, Ingrid Tessmer2, Andres Vera-Gomez1, and Izabela Kaminska1 — 1Chemistry Department and Center for NanoScience, Ludwig-Maximilians-Universität München — 2Rudolf Virchow Center, University of Würzburg, Würzburg
Obtaining structural information from single molecules is commonly associated with Fluorescence Resonance Energy Transfer that typically yields one distance between a fluorescent donor and an acceptor. Energy transfer to graphene with graphene-on-glass coverslips can extend the dynamic range to more than 30 nm. Based on the discovery that DNA can be placed vertically on graphene, we developed GETvNA (graphene energy transfer with vertical nucleic acids) that enables Angstrom precise visualization of DNA conformations and protein-DNA complexes. We envision that the alignment of DNA will additionally make it amenable to combined energy transfer and superresolution interrogation for dynamic structural biology.
Keywords: superresolution microscopy; single-molecule detection; fluorescence resonance energy transfer; graphene; DNA protein interactions