Regensburg 2025 – wissenschaftliches Programm
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BP: Fachverband Biologische Physik
BP 8: Biomaterials, Biopolymers and Bioinspired Functional Materials I (joint session BP/CPP)
BP 8.2: Vortrag
Montag, 17. März 2025, 15:15–15:30, H46
Highly sensitive, specific and label-free detection of SARS-CoV-2, Influenza A and RSV proteins via surface plasmon resonance technique using the biofunctionalization with 1 nm thick carbon nanomembranes — •Ghazaleh Eshaghi1, David Kaiser1, Hamid Reza Rasouli1, Rania Ennaciri1, Martha Frey1, Christof Neumann1, Dominik Gary2, Tobias Fischer2, Katrin Frankenfeld2, and Andrey Turchanin1 — 1Institute of Physical Chemistry, Friedrich Schiller University Jena, 07743 Jena, Germany — 2Forschungszentrum für Medizintechnik und Biotechnologie (fzmb) GmbH, 99947 Bad Langensalza, Germany
Accurate and rapid detection of respiratory viruses like SARS-CoV-2, Influenza A and RSV is crucial for improving global health outcomes. We present a novel surface plasmon resonance (SPR) platform using a biofunctionalized 1 nm-thick carbon nanomembrane (CNM) for enhanced viral protein detection. The azide-modified CNM (N3-CNM) enables covalent antibody binding, ensuring selective immobilization of target proteins. Our platform achieves equilibrium dissociation constants (KD) of 570 * 30 pM and 22 * 3 pM for SARS-CoV-2 nucleocapsid and spike proteins, with detection limits (LODs) of ~190 pM and ~10 pM, respectively. For Influenza A and RSV, KD values are 86 * 4 pM and 3 * 0.2 pM, with LODs of ~90 pM and ~2 pM. Multiplexed detection with no cross-reactivity supports rapid, accurate point-of-care diagnostics. Validation with nasopharyngeal swabs confirms a LOD of ~40 pM for SARS-CoV-2 spike protein, highlighting CNMs' promise in infectious disease diagnostics.
Keywords: SARS-CoV-2; surface plasmon resonance; two-dimensional materials; carbon nanomembrane; biosensors