Regensburg 2025 – wissenschaftliches Programm

Bereiche | Tage | Auswahl | Suche | Aktualisierungen | Downloads | Hilfe

CPP: Fachverband Chemische Physik und Polymerphysik

CPP 15: Poster Session I

CPP 15.16: Poster

Montag, 17. März 2025, 19:00–21:00, P4

Porous poly(ethylene glycol) films as a versatile platform for ssDNA immobilization and hybridization — Zhiyong Zhao and •Michael Zharnikov — Angewandte Physikalische Chemie, Universität Heidelberg, 69120 Heidelberg, Germany

Poly(ethylene glycol) (PEG) films, fabricated by thermally induced crosslinking of amine and epoxy-terminated four-arm STAR-PEG precursors, were used as porous and bioinert matrix for single-stranded DNA (ssDNA) immobilization and hybridization. The immobilization relied on either the reaction between the amine groups in the PEG matrix and N-hydroxy succinimide (NHS) ester groups of the NHS-ester-decorated ssDNA or the reaction between the epoxy groups in the matrix and thiol groups of the respectively substituted ssDNA. The mixing ratio of the precursors was varied to tune the density of the amine and epoxy groups available for the immobilization. Spectroscopic and electrochemical data confirmed the successful immobilization of the ssDNA probes into the PEG matrix as well as the high hybridization efficiency, selectivity, and sensitivity of the resulting DNA sensors. Whereas these sensors were equivalent to the direct ssDNA assembly in terms of efficiency, they exhibited better selectivity and bioinert properties because of the bioinert character of the PEG matrix. The above findings place PEG films as a promising platform for highly selective ssDNA sensing, leveraging their flexible chemistry, 3D character, and bioinert properties.

Keywords: Poly(ethylene glycol); bioinertness; biosensors; single-stranded DNA