Regensburg 2025 – wissenschaftliches Programm
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CPP: Fachverband Chemische Physik und Polymerphysik
CPP 15: Poster Session I
CPP 15.20: Poster
Montag, 17. März 2025, 19:00–21:00, P4
Analysis of folding/unfolding behavior of micro and nano collagen fibers by SFTIRM — •Selcuk Kaan Haciosmanoglu1, Gihan Kamel2, 3, and Murat Kazanci1, 4 — 1Nanoscience and Nanoengineering Program, Graduate School, Istanbul Medeniyet University, Istanbul, Turkey — 2SESAME (Synchrotron-light for Experimental Science and Applications in the Middle East), Allan, Jordan — 3Department of Physics, Faculty of Science, Helwan University, Cairo, Egypt — 4Biomedical Engineering Department, School of Engineering and Natural Sciences, Istanbul Medeniyet University, Istanbul, Turkey
Collagen nanofibers are essential extracellular matrix (ECM) components widely used in regenerative medicine. While both electrospinning and wet-spinning techniques can produce anisotropic collagen micro- and nanofibers from dissolved collagen solutions, electrospun fibers require crosslinking to maintain water stability for cell culture applications. This study investigates in-situ crosslinking during electrospinning using chemical agents and physical methods. Synchrotron Fourier-Transform Infrared Microspectroscopy (sFTIRM) analysis revealed distinct molecular structural changes in the collagen nanofibers based on processing methods. Protein band positions shifted according to the extraction methods employed. Notably, electrospinning inhibited collagen molecule self-assembly, resulting in lower band positions compared to wet-spun fibers. The choice of crosslinking agent significantly influenced collagen's secondary structure, with genipin-mediated in-situ crosslinking better preserving the native structure of electrospun collagen nanofibers compared to UV crosslinking.
Keywords: collagen; nanofiber; microfiber; sFTIRM