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CPP: Fachverband Chemische Physik und Polymerphysik
CPP 15: Poster Session I
CPP 15.23: Poster
Montag, 17. März 2025, 19:00–21:00, P4
How do stiffness patterns, with no topographical or chemical cues, influence cellular and tissue contact guidance? — •Mathis Grelier1, Carlos Urena Martin2, Mark Schvartzman2, and Ana Suncana smith1,3 — 1Puls Group, Institute for Theoretical Physics and Interdisciplinary Center for Nanostructured Films (IZNF), Friedrich-Alexander Universität Erlangen-Nürnberg (FAU), 91058 Erlangen, Germany — 2Department of Materials Engineering and Ilse Katz Institute for Nanoscale Science and Technology, Ben-Gurion University of the Negev, Beer-Sheva, Israel — 3Group of Computational Life Sciences, Division of Physical Chemistry, Rudjer Bošković Institut, 10000 Zagreb, Croatia
While current systems cannot disentangle the effects of topography and stiffness contrasts, our study overcomes this challenge by investigating cell and tissue behavior on substrates with only alternating stiffness stripes. Our experiments and model reveal that wider stripes enhance alignment along stiffer or softer regions due to the higher energy cost of traversing softer substrates. At the cellular level, we employ a stochastic model based on Boltzmann weights applied to possible cell shapes, integrating energy contributions that govern movement across stiffness boundaries. For tissues, we extend this framework using a dissipative particle model to capture collective dynamics and mechanical interactions. To further explore these effects, we applied a Globalized Bounded Nelder-Mead optimization, enabling the efficient recovery of key physical parameters, such as stiffness and contractility, under both untreated and treated conditions.
Keywords: Substrate Stiffness; Contact Guidance; Dissipative Particle Model; Cell Migration Biomechanics