Regensburg 2025 – wissenschaftliches Programm

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MM: Fachverband Metall- und Materialphysik

MM 29: Liquid and Amorphous Materials

MM 29.2: Vortrag

Donnerstag, 20. März 2025, 12:15–12:30, H10

Peptide Analysis at Atomic Resolution with Atom Probe Tomography — •Sakshi Sinha and Guido Schmitz — Department of Materials Physics, Institute of Material Science, University of Stuttgart, Heisenbergstr. 3, 70569 Stuttgart

Atom Probe Tomography (APT) promise decisive advancements in nanoscale characterization of biomolecules, since it offers in principle single atom sensitivity in the study of organic molecular structures. In our experiments, we test the analysis of carnosine (β-alanylhistidine), in aqueous solution since water provides a natural biological environment. The investigated dipeptide is for example essential for brain and muscle function. By combining cryogenic sample preparation with advanced laser-pulsing techniques, APT enables three-dimensional mapping of carnosine's atomic composition and the distribution of various molecule fragments in the water matrix. Remarkably, the concentration of the solution controls the detected fragmentation behavior in the full range from single atoms to the full peptide molecule. So, the measurement conditions can be optimized to preferentially address the questions regarding the local stoichiometry or the different subunits of the molecule.

Keywords: Atom Probe Tomography; Three-Dimentional atomic mapping; Fragmentation